HPLC Column, RP PFP, 5um, 4.6mm ID x 250mm Length, 60A. Cogent Brand. 1 EA.
Additional Info:
60022-25P Cogent RP PFP™ HPLC Column has been a premium value trusted by HPLC laboratories for many years, delivering reliable performance and enhanced selectivity for complex separations. This column is fully double end‑capped and bonded via a four‑carbon linker to a benzene ring substituted with five fluorine atoms, using ultra‑high purity silica.
The column provides multiple retention mechanisms—including π‑π interactions, dipole interactions, and hydrogen bonding—making it highly effective for halogenated, aromatic, and polar analytes. It is also suitable as an alternate column for USP methods requiring L43 classification.
With a 5 µm particle size, this column offers lower backpressure compared to smaller particle columns, allowing higher flow rates and robust, reproducible performance in standard analytical HPLC systems.
250 mm Length – Maximum Resolution for Complex Separations
The 250 mm length provides the highest resolving power, making it ideal for demanding applications involving complex mixtures or closely related compounds.
Maximum resolution compared to shorter column lengths
Superior separation of co-eluting and structurally similar analytes
Increased peak capacity for complex matrices
✅ Recommended for impurity profiling, method development, and high-resolution analytical work
Flow Rate & System Requirements (4.6 mm ID, 5 µm)
⚠️ Fully compatible with standard analytical HPLC systems with manageable backpressure
Typical flow rate range: ~1.0 – 1.5 mL/min
Lower backpressure than 3 µm columns
Supports stable, higher flow rate operation
Compatible with UV and standard detectors
✅ Best Practice: Filter samples using a 0.2 µm syringe filter prior to injection
Why Choose PFP (F5) Columns for Selective Separations
Enhanced Retention for Halogenated Compounds
Strong interaction with fluorinated and halogenated analytes
Improved selectivity vs C18 columns
Multiple Interaction Mechanisms
π‑π interactions for aromatic compounds
Dipole and hydrogen bonding interactions
Greater flexibility in method development
60 Å Pore Structure
Optimized for small molecule separations
Efficient interaction for lower molecular weight analytes
Cogent RP PFP™ Column Advantages
Fully End‑Capped Surface
Minimizes silanol activity
Improves peak symmetry
Robust Performance (5 µm Particles)
Lower backpressure compared to smaller particle sizes
Reliable and consistent operation
High Purity Silica
Reproducible chromatographic performance
Long column lifetime
Typical Applications
Halogenated compound analysis
Aromatic compound separations
Pharmaceutical QA/QC workflows
Method development and impurity profiling
USP L43 methods
Important Usage Notes
Packed and shipped in reversed‑phase solvents
Designed for standard analytical HPLC systems (4.6 mm ID)
Lower backpressure compared to smaller particle sizes
Use 0.45 µm filtration prior to injection